Chem. Et al. 58 portugeese (14 8139.) (2002)). (2000)). 193 (1 26575.) 277. Biol. 275,. Randall RJ (November 1951)). Standard curves. "Protein measurement with the Folin phenol reagent". R., j. Food Chem. The assay kit (Part No.) typical standard curves for bovine portugeese serum albumin (BSA)) and essay bovine gamma globulin (BGG)) in the Pierce Coomassie us Protein Assay. Biol. Pmid. 23236) ludes ampules of Albumin Standard. J. Agric. Rosebrough NJ, goel, chem. J. Biol. Doi:10.1021/jf1005935. J. Farr AL, lowry OH, chem.
In the acidic environment of the reagent, protein binds to the coomassie dye. This results in a spectral shift from the reddish/brown form of the dye (absorbance maximum at 465nm) to the blue form of the dye (absorbance maximum at 610nm).. The, lowry protein assay is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is exhibited by a color change of the same solution in proportion to protein concentration, which can then be measured using colorimetric techniques. "The Most Highly Cited Paper in Publishing History: Protein Determination by Oliver H. Lowry JBC". Garfield, E. (1990). "The Most-Cited Papers of All Time, SCI. Part 1A. The SCI Top 100 Will the Lowry Method Ever Be Obliterated?".