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The Pierce Coomassie us Assay Reagent provides reased linearity of response and only half the protein-to-protein variability of other mercial Bradford assay formulations. Highlights: Colorimetric measure with a standard spectrophotometer or ate reader (595nm easy to use single reagent; no working reagent preparation required, fast almost immediate color development; , mix and read results, broad range detects protein concentration in the range 1 to 1500g/mL, better improved linearity and response-uniformity pared to traditional Bradford formulations, flexible microate and cuvette protocols provided with the instructions and adaptable to several target working ranges, product Details: Video of the Pierce Coomassie.. In the acidic environment of the reagent, protein binds to the coomassie dye. This results in a spectral shift from the reddish/brown form of the dye (absorbance maximum at 465nm) to the blue form of the dye (absorbance maximum at 610nm). Judith Sargent Murray, despite of being born into a wealthy family on March 01, 1751 in Gloucester, Massachusetts, did not receive many opportunities for a formal education beyond reading and writing.