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What Does the Essay Look Like? What follows is a testlike exame of a Student Response, sheet. Weve inserted numbered boxes that ment on each part of the Student Response Sheet.. Pmid. External links edit). At the two extremes (575nm and 615nm) there is a loss of about 10 in the measured amount of color (absorbance) pared to that obtained at 595nm. Development of color in coomassie dye-based (Bradford) protein assays has been associated with the presence of certain basic amino acids (primarily arginine, lysine and histidine) in the protein.